oligonucleotides, primers, and synthetic genes (GenScript corporation)
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Oligonucleotides, Primers, And Synthetic Genes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Virus:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Recombinant:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Staining:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Membrane:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Thin Layer Chromatography:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Polymerase Chain Reaction:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and Purification:Article Title: Prokaryotic Gabija complex senses and executes nucleotide depletion and DNA cleavage for antiviral defense. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli DH5a Sangon Biotech Cat#B528413 E. coli BL21(DE3) Yeasen Cat#11804ES80 E. coli B (ATCC 11303 ) American Type Culture Collection (ATCC) ATCC 11303 Bacillus subtilis (ATCC 6051 ) American Type Culture Collection (ATCC) ATCC 6051 Phage T4 China Center For Type Culture Collection (CCTCC) Genbank: AF158101.6 Phage T5 China Center For Type Culture Collection (CCTCC) Genbank: AY543070.1 Phage T7 China Center For Type Culture Collection (CCTCC) Genbank: NC_001604.1 Chemicals, peptides, and recombinant proteins Kanamycin Sigma Cat#K0254 Ampicillin Sigma Cat#A9518 NTPs (ATP, GTP, UTP, CTP) New England Biolabs Cat#N0450S DNA stain DAPI Sangon Biotech Cat#E607303 Membrane stain FM4-64 Thermo Fisher Scientific Cat#T3166 Ni-NTA Agarose QIAGEN Cat#30250 Preparative Superdex S200 column GE Healthcare Cat#17-1043-01 Silica Gel-60 F254 glass-backed TLC plate MilliporeSigma Cat#M1057290001 30% Acrylamide/Bis Solution, 37.5:1 Bio-Rad Cat#1610158 Tris base Roche Cat#10708976001 Imidazole Sigma-Aldrich Cat#IX0005 Dithiothreitol (DTT) Sigma-Aldrich Cat#D0632 Chloroform Sigma Cat#C2432 IPTG (Isopropyl-b-D-thiogalactoside) Sigma Cat#10724815001 GajA recombinant protein, and mutants as described This paper N/A GajB recombinant protein, and mutants as described This paper N/A Critical commercial assays QIAquick PCR Purification Kit QIAGEN Cat#28106 PiColorLock phosphate detection system kit Expedeon Cat#303-0030 DNeasy blood and tissue kit QIAGEN Cat#69504 RNase A QIAGEN Cat#27106 DNase-I New England Biolabs Cat#M0303 Gibson assembly kit New England Biolabs Cat#E2611 RNA purification kit New England Biolabs Cat#E2611 PrimeSTAR Max DNA Polymerase TaKaRa Cat#R045A Deposited data RNA-Seq data This paper SRA: PRJNA961899 Oligonucleotides, primers, and synthetic genes The sequences are listed in Tables S1, S2, and |
